Table 4.
IC50 values (72 h mean ± SD, µM), combination index and fold reversal of 5FU, LY294002, HAA2020, and their combinations in HCT116 and HCT116-5FU cells.
| Drug(s) (Ratio) | HCT116 | HCT116-5FU | |||||
|---|---|---|---|---|---|---|---|
| IC50 | CI | r | IC50 | CI | r | FR | |
| 5FU | 0.19 ± 0.03 | - | 0.90 | 44.00 ± 5.10 | - | 0.93 | - |
| LY294002 | 11.54 ± 01.22 | - | 0.92 | 39.34 ± 5.12 | - | 0.96 | - |
| HAA2020 | 4.11 ± 0.50 | - | 0.98 | 13.33 ± 0.65 | - | 0.90 | - |
| 5FU: LY294002 (1:1) | 3.16 ± 0.67 | 12.87 | 0.91 | 40.23 ± 4.10 | 17.70 | 0.92 | 1.1 |
| 5FU: HAA2020 (1:1) | 0.15 ± 0.03 | 0.90 | 0.95 | 8.00 ± 1.12 | 0.95 | 0.90 | 5.5 |
| LY294002:HAA2020 (1:1) | 6.01 ± 0.89 | 5.11 | 0.89 | 30.88 ± 3.40 | 9.11 | 0.91 | - |
| 5FU: LY294002: HAA2020 (1:1:1) |
3.76 ± 0.41 | 2.20 | 0.96 | 25.11 ± 3.00 | 17.00 | 0.92 | 1.7 |