Table 5.
IC50 values (72 h mean ± SD, µM) and selectivity index of 5FU, LY294002, HAA2020, and their combinations in MRC5 cells. Experiments were repeated ×3 (n = 3).
| Drug(s) (Ratio) | IC50 | SI d | |||
|---|---|---|---|---|---|
| MRC5 | HT29 | HT29-5FU | HCT116 | HCT116-5FU | |
| 5FU | 30.91 ± 4.22 | 134.4 | 0.4 | 162.6 | 0.7 |
| LY294002 | 28.65 ± 2.56 | 3.2 | 0.9 | 2.5 | 0.7 |
| HAA2020 | 19.44 ± 1.99 | 5.2 | 2.1 | 4.7 | 1.4 |
| 5FU: LY294002 (1:1) | 12.51 ± 1.40 | 31.2 | 0.2 | 3.9 | 0.3 |
| 5FU: HAA2020 (1:1) | 10.30 ± 0.78 | 206.0 | 1.1 | 68.6 | 1.2 |
| LY294002:HAA2020 (1:1) | 8.79 ± 1.34 | 9.6 | 0.4 | 1.5 | 0.3 |
| 5FU: LY294002: HAA2020 (1:1:1) |
5.40 ± 0.94 | 60.0 | 0.3 | 1.4 | 0.2 |
d, SI: selectivity index = IC50 value of a compound against either normal MRC-5 or HUVEC cells/IC50 value of the same compound or combination against either HT29, HT29-5FU, HCT116, or HCT116-5FU cells. Experiments were repeated ×3 (n = 3).