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. 2021 Jan 9;22(2):597. doi: 10.3390/ijms22020597

Figure 4.

Figure 4

NDPK inhibition and P2Y1 receptor antagonism reverse MDA-MB-231 EV-mediated vascular endothelial cell migration. (A) Quantitation of HUVEC migration through collagen-coated transwells following a 24-h treatment with MDA-MB-231 EVs or HME1 EVs. Scale: 200 μm. n = 3. Mean ± S.E.M. ** p < 0.01 by nonparametric two-tailed Student’s t-test. (B) HUVEC migration through collagen-coated transwells following a 24-h treatment with complete growth medium (VL-VEGF), low serum (VL-2% FBS), MDA-MB-231 EVs (MDA-231 EV) with and without MRS2500, MRS2270, MRS2179, ellagic acid (EA), and ellagic acid with MRS2179. Scale: 200 μm. n = 3. Mean ± S.E.M. *** p < 0.001 by one-way ANOVA and Tukey’s post-test. (C) MLEC migration through collagen-coated transwells following a 24-h treatment with complete growth medium (EGM-2), VEGF, MDA-MB-231 EVs, and EVs with ellagic acid and MRS2179. Scale: 200 μm. n = 3. Mean ± S.E.M. * p < 0.05, ** p < 0.01 by one-way ANOVA and Tukey’s post-test.