Table 2.
Most reactive aminoacidic sequences selected by the high-density microarray and results of the validation performed with progressively larger patients cohorts. The peptide sequences were analyzed in terms of fluorescence intensity of IgG immune-reactivity of Covid-19 positive sera and discrimination with the healthy control group by the unpaired t test. The p values of the screenings are reported.
| Protein | Code | Sequence |
p Value 1st Screening N = 12 |
p Value 2nd Screening N = 28 |
|---|---|---|---|---|
| Orf1ab | AM48 | CASYQTQTNSPRRAR | 0.2958 | |
| S | AM49 | YQAGSTPCNGVEGFN | 0.1942 | |
| S | AM50 | FDNPVLPFNDGVYFA | 0.0180 | 0.0101 |
| Orf1ab | AM51 | LGVYDYLVSTQEFRY | 0.1499 | |
| Orf1ab | AM52 | QDGNAAISDYDYYRY | 0.0470 | 0.3314 |
| Orf1ab | AM54 | GLPGTILRTTNGDFL | 0.2074 | |
| S | AM55 | CASYQTQTNSPRRAR | 0.0359 | 0.2882 |
| Orf1ab | AM56 | IDLVPNQPYPNASFD | 0.5966 | |
| N | AM57 | AIVLQLPQGTTLPKG | <0.0001 | <0.0001 |
| S | AM63 | SNLKPFERDISTEIY | 0.0249 | 0.2640 |
| Orf1ab | AM65 | DNQDLNGNWYDFGDF | 0.0115 | 0.0317 |
| N | AM66 | GNFGDQELIRQGTDY | 0.0771 |