Figure 10.

Cell nuclei staining using Hoechst 33342 in HaCaT cells after treatment with Rut (20 and 50 µM) and DMSO for 24 h. The pictures were taken 24 h posttreatment. Staurosporine solution (5 µM) represents the positive control for apoptotic changes at the nuclear level and Triton X-100 solution (0.5%) for necrosis. The yellow arrows indicate apoptotic cells with nuclear fragmentation, and the red arrow indicates a necrotic cell. The scale bars represent 20 µm.