Figure 4.

The effect of lidocaine on TRPM7 channels in breast cancer cell lines. (A–D). Fluorescence quench assay (n = 3): The fluorescence quenches followed by the addition of Mn²⁺ were plotted on the same scale. 10 µM Gd²⁺ was used as the negative control. (E–H). The average fluorescence quench amount of 200–300 s in MDA-MB-231, AU565, T47D, and MDA-MB-468. “*” indicates the significant differences (p < 0.05) compared with the control. The quench data of the vehicle, negative, and 200 µ 2-APB in MDA-MB-231 were published previously [22].