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. 2021 Jan 10;9(1):143. doi: 10.3390/microorganisms9010143

Figure 1.

Figure 1

Confocal microscopy images of living Candida albicans cells treated with fluorescein isothiocyanate (FITC)-labeled “killer peptide” (KP). After 170 min of treatment, KP localized on the yeast cell surface (A). All yeast cells were viable, as assessed by propidium iodide exclusion (B). (C) merge of panels A and B. After 380 min of treatment, KP entered yeast cells (D). Some yeast cells were no longer viable, as assessed by propidium iodide internalization (E). (F) merge of panels D and E. Bar: 5 µm (panels A, B, and C); 2 µm (panels D, E, and F). The slow killing effect suggests a non-membranolytic mode of action.