Figure 5.

Effects of Abeliophyllum distichum leaf extract on LPS-induced ROS production in RAW264.7 macrophages. The inhibitory effect of A. distichum leaf (AL) or branch (AB) extract on LPS-induced ROS production was determined using the DCFH-DA (A). LPS-treated RAW264.7 cells in the presence of AL were used to analyze the expression level of genes involved in ROS production (B), cellular oxygen metabolism (C), and Nrf2-targeted antioxidant and detoxification (D). The transcript levels of each gene was normalized to β-actin and expressed relative to the value of LPS alone. All data were reported as the mean ± SE. Different superscripted letters indicate the significant differences (p < 0.05).