Figure 6.

Repressive effects of ULH-002 on UVA-induced cell death and collagen synthesis decline in three-dimensional skin equivalents. The 3D skin equivalents were constructed as described in the ‘‘Materials and Methods’’. (A), The 3D skin equivalents were pretreated with ULH-002 at 0.3 mg/mL for 24 h. The equivalents were then irradiated with UVA (32 J/cm²). At 24 h after UVA irradiation, the expression of type IV and I collagens was observed by immunostaining. (B), The immunostaining results were analyzed with an ImageJ software. NC, negative control group (sham irradiation); PC, positive control group. Data were expressed as mean ± SD. **, p < 0.01, ***, p < 0.001 vs. PC; blue, nuclei; red, type IV or I collagen. Scale bar = 100 µm.