Figure 3.

ZIKV infection of mature CNS cultures was accompanied by myelin damage and axonal injury. Representative images of mature mouse CNS cultures infected with ZIKV on DIV 26 with MOI 0.3 (n = 3). Images taken at 2 dpi (DIV 28), 4 dpi (DIV 30), and 6 dpi (DIV 32). Upper panels show mock-infected and lower panels show ZIKV-infected samples. (A) ZIKV-infected cells (ZIKV E staining, green signal), and oligodendrocyte staining as determined by co-labeling with PLP (red signal). Insets are enlarged areas of the presented image. White rectangles show the origin of the enlarged area. Scale bar = 100 µm. (B) Myelin sheaths visualized by MBP staining (green signal). Scale bar = 100 µm. Insets show high magnification images of myelin; scale bars = 10 µm. (C) Axonal damage was observed at 4 dpi (the white arrow highlights the damaged axon enlarged in the white rectangle); axonal density visualized by neurofilament (NF) staining (red signal). Neuronal cell bodies (NeuN staining, green signal) showing signs of injury by accumulating neurofilament (yellow arrows). Scale bar = 100 µm. Insets in images of ZIKV-infected cultures show high-magnification images of axons and neuronal cell bodies with white arrows indicating axonal injury; scale bar = 10 µm.