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Protective effect of Rut on APAP-induced NF-κB activation in mice. Hepatic protein levels of phospho-NF-κB p65, NF-κB p65, phospho-IκBα, and IκBα were determined using western blotting (A,B). Protein level was analyzed using ImageJ software. Relative expression of the target protein was compared using β-actin as a control. (C,D) are indicated as means ± SD (n = 10). # Significantly different from the control (p < 0.05). * Significantly different from the APAP-treated group (p < 0.05).
