Figure 5.

Viability of mouse peritoneal cells. Isolated cells were cultured for 24 h. Individual compounds, i.e., ouabain (Ob), cymarin (Cy), hyrcanoside (Hyr), and deglucohyrcanoside (deHyr), were applied at concentrations of 0.001, 0.01, 0.1, 1.0, 10.0, 50.0, and 100 µM. The effect of DMSO was also analyzed; its concentration corresponded to 50 µM concentration of compounds. WST-1 assay was used for viability evaluation. The results are expressed as the percent of untreated controls (ctrl) ± SEM (standard error of the mean) of n = 8 values from two independent experiments.