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Effect of epiremisporine E (3) on the colony formation of A549 cells. (A) The effect of 3 against A549 cell colony formation. Clonogenicity was evaluated by the monolayer colony formation assay. Representative images show the blue colonies of A549 cells stained with crystal violet. (B) Histogram presentation of A549 cell colony quantification. * p < 0.05; *** p < 0.001 compared with the control. a 5-Fluorouracil (5-FU) was used as a positive control.
