Table 5.
Results of PNT in mice.
| Ref. | Transfer Method | Blastocyst Development | Births Rate | Carry-Over | Notes |
|---|---|---|---|---|---|
| McGrath et al., 1983 [42] | EF | 96% (64/67) Similar to controls |
15% (10/64) Similar to controls |
Not tested | First publication about PNT |
| Sato et al., 2005 [43] | EF | Not indicated | 28% (11/39) Similar to controls |
F1 tail: 11% (6–21%) After 300 days: 23% (5–44%) |
Murine model with mitochondrial pathology. Offspring free of disease |
| Cheng et al., 2009 [27] | EF | Not indicated | 31–62% | Not tested | Animals born were healthy and comparable to controls |
| Neupane et al., 2014 [28] | HVJ-E | 87.5% (14/16) Similar to non-manipulated controls |
Not tested | Embryos: 0.29% ± 0.75% Undetectable in 21/25 |
Minimum levels of carry-over. Equivalent to those of GVT and MST |
| Wang et al., 2014 [31] | HVJ-E | 81.3% (13/16) Similar to controls |
53% (7/13) Similar to controls |
F1 tail: 23.7% ± 11.1% F2 fingers: 22.1% ± 18.7% |
High rates of carry-over, probably due to manipulation problems |
ESCs: embryonic stem cells. EF: electrofusion. F1/F2: mice of first and second generation. HVJ-E: extract of de Sendai virus. ICSI: intracytoplasmatic spermatozoid injection.