. 2021 Jan 7;22(2):551. doi: 10.3390/ijms22020551

Table 7.

Results of PBT in animals and humans.

Ref.	Species	Transfer Method		Blastocyst Develop	Births Rate	Carry-Over	Notes
Wang et al., 2014 [31]	Mice (NZW and BDF1)	HVJ-E	PB1T	82.4% (14/17) similar to MST	42.8% (6/14) similar to MST	F1 tail: 0% (0/6) F2 fingers: 0% (0/16) lower to MST	Compared PB1T with MST and PB2T with PNT
Wang et al., 2014 [31]	Mice (NZW and BDF1)	HVJ-E	PB2T	40% (6/15) lower than PNT	40% (6/15) similar to PNT	F1tail: 1.7 ± 2.8% F2 fingers: 2.9 ± 4.3% lower than PNT	Compared PB1T with MST and PB2T with PNT
Ma et al., 2017 [39]	Humans	HVJ-E	PB1T	32.5% (8/25) lower than control	Not tested	Not tested	Same group of refs: [19,30,33]
Zhang et al., 2017 [38]	Humans	HVJ-E	PB1T	24.1% (7/29) similar to control	Not tested	Not tested	Data of fresh and cryogenized karyoplast transfers were added up
Zhang et al., 2017 [38]	Humans	HVJ-E	PB2T	0% (0/17) lower than control	Not tested	Not tested
Wu et al., 2017 [40]	Humans	HVJ-E	PB1T	25.3% (19/75) similar to control and PB2T	Not tested	Embryos: 0.26% (0.07–0.5%) ESCs: 0.15% (P5-P20)	Good results due to performing the transfer right after the fertilization (pre-pronucleus) Same group of ref. [37]
Wu et al., 2017 [40]	Humans	HVJ-E	PB2T	27.5% (14/51) similar to control and PB1T	Not tested	Embryos: 0.37% (0.06–0.7%) ESCs: 0.22% (P5-P20)

HVJ-E: Sendai virus extract. PB1T: first polar body transfer. PB2T: second polar body transfer. MST: meiotic spindle transfer. PNT: pronucleus transfer. ESCs: embryonic stem cells. F1: first generation. F2: second generation.