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. 2021 Jan 12;13(2):259. doi: 10.3390/cancers13020259

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Induction of MET by SPCA2 is independent of Ca²⁺-ATPase activity. (A,B) Ectopic expression of SPCA2 mutant D379N in MDA-MB-231 significantly decreased vimentin compared to control, as determined by (A) qPCR (n = 3) or (B) Western blotting. GAPDH was used as a loading control. Quantified by densitometry (n = 3). (C–F) Mesenchymal gene markers, measured by qPCR, were decreased following ectopic expression of SPCA2R D379N as shown. n = 3. (G–J) Representative live cell Ca²⁺ imaging baseline readings (10× magnification) in Ca²⁺-free conditions using Fura2-AM treated MDA-MB-231 cells with or without SPCA2R (G) or D379N (I). Fluorescence emission ratio of 340/380 nm showing increase in average resting Ca²⁺ in the presence of (H) SPCA2R (control n = 30 cells, SPCA2R n = 30 cells) or (J) D379N (control n = 40 cells, SPCA2R D379N n = 40 cells). Student t- test; *** p < 0.001, ** p < 0.01, * p < 0.05, ^ns p > 0.05.

Induction of MET by SPCA2 is independent of Ca²⁺-ATPase activity. (A,B) Ectopic expression of SPCA2 mutant D379N in MDA-MB-231 significantly decreased vimentin compared to control, as determined by (A) qPCR (n = 3) or (B) Western blotting. GAPDH was used as a loading control. Quantified by densitometry (n = 3). (C–F) Mesenchymal gene markers, measured by qPCR, were decreased following ectopic expression of SPCA2R D379N as shown. n = 3. (G–J) Representative live cell Ca²⁺ imaging baseline readings (10× magnification) in Ca²⁺-free conditions using Fura2-AM treated MDA-MB-231 cells with or without SPCA2R (G) or D379N (I). Fluorescence emission ratio of 340/380 nm showing increase in average resting Ca²⁺ in the presence of (H) SPCA2R (control n = 30 cells, SPCA2R n = 30 cells) or (J) D379N (control n = 40 cells, SPCA2R D379N n = 40 cells). Student t- test; *** p < 0.001, ** p < 0.01, * p < 0.05, ^ns p > 0.05.