Skip to main content
. 2021 Jan 12;9(1):152. doi: 10.3390/microorganisms9010152

Figure 6.

Figure 6

Effect of sub-inhibitory concentrations of aztreonam lysine (AzLys) on the motility of P. aeruginosa PA01. (A) PA01 grown in the presence or absence of sub-inhibitory AzLys concentrations were stab inoculated through 1% agar and incubated for 24 h at 37 °C. Twitching zones below the agar were visualized with TM developer solution and the diameter was measured using ImageJ [29]. (B) PA01 grown in the presence or absence of sub-inhibitory AzLys concentrations were stab inoculated midway through 0.3% ½ LB agar and incubated for 24 h at 37 °C. Diameter of swimming zone was measured using ImageJ. Representative twitching or swimming zones are shown on the left and the average diameter of each from nine replicates is shown on the right, including error bars showing standard deviation. Statistical analyses were performed using ANOVA and Dunnett’s post-test compared to the controls (***, p < 0.001; ****, p < 0.0001).