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. 2021 Jan 7;10(1):95. doi: 10.3390/cells10010095

Figure 5.

Figure 5

Puncta count analysis following 20 µM spermidine (H Sper) over 24 h in the presence (+Baf) and absence (−Baf) of bafilomycin. (A) Total GFP-LC3 puncta analysis. N = 24. * p < 0.05 vs. Con, # p < 0.05 vs. Con +Baf, @ p < 0.05 vs. 2 h +Baf, $ p < 0.05 vs. 8 h −Baf, % p < 0.05 vs. 8 h +Baf. (B) Total p62 puncta analysis. N = 12. * p < 0.05 vs. Con, # p < 0.05 Con +Baf, @ p < 0.05 vs. 2 h −Baf, $ p < 0.05 vs. 2 h +Baf, % p < 0.05 vs. 24 h −Baf. (C) Co-localised GFP-LC3/p62 puncta analysis. N = 12. * p < 0.05 vs. Con, # p < 0.05 vs. Con +Baf, @ p < 0.05 vs. 2 h +Baf, $p < 0.05 vs. 8 h −Baf. (D) Total NBR1 puncta analysis. N = 12. * p < 0.05 vs. Con, # p < 0.05 vs. Con +Baf, @ p < 0.05 vs. 2 h +Baf, $ p < 0.05 vs. 8 h +Baf. (E) Co-localised GFP-LC3/NBR1 puncta analysis. N = 12. # p < 0.05 vs. Con +Baf, @ p < 0.05 vs. 8 h +Baf. (F) Representative fluorescence micrographs showing GFP-LC3 and p62 puncta. (G) Representa-tive fluorescence micrographs showing GFP-LC3 and NBR1 puncta. Scale bar = 10 µm.