Figure 2.

Phage lysis of EV76::lux suspended in Brain Heart Infusion (BHI) or in whole blood. A bioluminescent lysis assay was performed with 90 µL of 10⁷ CFU/mL EV76::lux suspended in BHI (A) or in human whole blood (B) and infected with 10 µL of ϕA1122 or PST phages (10⁶ PFU/mL; MOI = 0.01). Bioluminescence was assessed by tracking the relative light units (RLU) at 37 °C in 15-min intervals for 24 h using a Spark 10 M plate reader. The experiment was performed using 10 different blood donors (of various blood types) and the results are representative of one blood donor (blood type O+, 9 days from uptake). Values are the average results from 3 wells triplicate in the same experiment, and the error bars represent the standard deviation (STDEV).