Figure 5.

Virus entry mediated by the gB N-glycosylation site mutants. RK13 cells were transfected with expression plasmids encoding WT or mutant gB. Cells transfected with an empty vector served as a negative control (Mock). 24 h later, the cells were infected with PrV-ΔgB at an MOI of 3. Progeny virus titers were determined on gB-expressing RK13 cells and are given in plaque-forming units (PFU)/ml. Shown are the mean values from four independent experiments and corresponding standard deviations.