Table 1.

Oligonucleotides used for mutagenesis and sequence analyses.

Primer Name	Sequence (5′➔3′)
gB Ka N154Q F	CTCGCAGGGGCGCCAGTTCACGGAGGGG
gB Ka N264Q F	CGGCTGGCACACCACCCAGGACACCTACACC
gB Ka N444Q F	CGGCGGCGCTACCAGAACACGCACGTGCTGG
gB Ka N519Q F	CGCCGGCCGTCCAGGGCACGGGGCACC
gB Ka N636Q F	CACCTTCGAGCACCAGGGCACGGGCGTG
gB Ka N700Q F	CGCGGGTGACCCTGCAGCTGACGCTGCTGG
130	CGTGCCCGTCCCCGTGCAGGAGATC
134	CCATCTACCGGCGGCGCTACAACAG

Only forward strand mutagenesis primers (F) are listed. The corresponding reverse strand primers are exactly complementary. PrV gB residues are numbered according to GenBank accession number EM64049.1 [64]. Nonmatching nucleotides are shown in boldface.