Figure 4.

Phylogenetic relationships, gene structure, and motif distribution of MaCsls. (A) Unrooted phylogenetic tree of 42 MaCsls, generated with the MEGA7.0 software by the neighbor-joining method with 1000 bootstrap replicates after alignment of the full-length protein sequence by Clustal W. (B) Exon/intron structures of MaCsl genes. (C) Conserved motifs of the MaCsl gene family, analyzed by Multiple Em for Motif Elucidation (MEME). Different motifs are represented by different colored boxes with numbers 1–10 (color figure online).