Figure 3.

Endothelial cell-specific deletion of CRIF1 causes mitochondrial dysfunction in the heart. (a) Western blot analysis for mitochondrial oxidative phosphorylation (OXPHOS) complex subunits in the heart tissues of mice. (b) OXPHOS subunits protein levels were quantified by densitometric analysis. (c) Tetramethyl rhodamine ethyl ester (TMRE) and (d) cardiac ATP levels were determined by using a colorimetric/fluorometric assay kit. (e) Mitosox-red fluorescence intensity. (f) Relative 2-hydroxyethidium (2-EOH) fluorescence intensity were measured by fluorescence reader. All data are presented as means ± SEM of at least three independent experiments (n = 5 mice in each group; * p < 0.05 vs. WT group).