Figure 4.

Decreased sirtuin 1 (SIRT1) expression and activation in CRIF1 EKO mouse hearts. (a) SIRT1 deacetylase activity in heart homogenates. (b) SIRT1 mRNA levels in the heart tissues. (c) Cardiac endothelial cells were separated from the heart tissues using anti-CD31 and anti-CD45 magnetic beads. Endothelial (CD31+/CD45−) and non-endothelial (CD31−/CD45+) SIRT1 protein expression were determined by Western blotting. α-tubulin was used as the internal control. The data are presented as means ± SEM of at least three independent experiments. (n = 5 mice in each group; * p < 0.05 vs. WT mice).