Figure 5.

I. Tomographic reconstruction of HPF Vero cells at 18 hpi. (a,b) Two slices from the tomogram in supplemental data (Video S1). High-pressure freezing reveals the existence of small membrane fragments (pink arrows) and the clarity afforded by tomography shows the capsid and core proteins accumulating on the outer and inner faces of the later membrane assembly intermediates (blue arrows). The area within the black box in (a) was modeled in IMOD and then viewed from two different angles (c,d). Small, apparently individual, membrane fragments can be seen (pink arrows) alongside slightly larger curved membranes (light blue arrows) with a connection visible between curved pre-virions (white arrows). When viewed from a different angle, the membrane fragments appear to form links or bridges between larger intermediates (dark blue arrows). Scale bars = 200 nm.