Figure 2.

US2-mediated HLA class I expression is rescued upon knockout of multiple players in the UFMylation pathway. (A) Schematic overview of the UFMylation cycle. UfSP2 cleaves UFM1 downstream of its C-terminal glycine, either to create active UFM1 from its precursor, or to release UFM1 from a substrate. UFBP1 is a target of UFMylation but may also aid the UFMylation reaction. (B) Individual genes involved in UFMylation were targeted by two sgRNAs (circles and diamonds) and monitored for rescued HLA-A2-eGFP expression at 12 (black) or 14 (grey) days post-infection. UFSP1 was included as negative control, as this protein is only functional in murine cells but not in human tissue. The experiment was performed three times, of which one time point each of two independent experiments is shown. (The two time course experiments are shown in Figure S2). (C) Flow cytometry plots of the data shown in B for day 12.