Table 1.
Results of linearity regression, correlation coefficient, LOD, and LOQ for quercetin-3-O-glucuronide and ellagic acid.
| Standard | Regression Equation | R2 | Residual STD (σ) | Calibration Curve Slope (S) | LOD (μg/mL) | LOQ (μg/mL) |
|---|---|---|---|---|---|---|
| Quercetin-3-O-glucuronide | 33.9200χ + 16.3234 | 1.0000 | 5.0 | 34.0 | 0.5 | 1.5 |
| 33.9749χ + 16.3269 | 1.0000 | |||||
| 33.9666χ + 7.6711 | 1.0000 | |||||
| Integration (n = 3) | 33.9538χ + 13.4405 | 1.0000 | ||||
| Ellagic acid | 118.7395χ + 322.2313 | 0.9997 | 36.3 | 119.4 | 1.0 | 3.0 |
| 120.2432χ + 249.6622 | 0.9999 | |||||
| 119.2289χ + 285.3886 | 0.9998 | |||||
| Integration (n = 3) | 119.4039χ + 285.7607 | 0.9998 |
The mean ± standard deviation of peak retention time was 19.6 ± 0.1 min for quercetin and 22.3 ± 0.1 min for ellagic acid, respectively. σ, residual standard deviation; S = calibration curve slope; Limit of detection (LOD) = 3.3 × σ/S; Limit of quantification (LOQ) = 10 × σ/S.