Figure 4.

Effects of the different concentrations of the phyto-fungicide on zebrafish eleutheroembryo motor behaviour. (A) Representative swimming tracks of untreated and treated zebrafish eleutheroembryo at 120 hpf. No significant changes were observed between the different treatments and the control group during the 10 min recording period. (B) Avoidance behaviour of the zebrafish eleutheroembryo in the presence of an aversive stimulus. Data is expressed as mean ± SD from five independent replicates (5 eleutheroembryo assayed for each treatment). Statistical analysis was performed using t-test: * p < 0.05, ** p < 0.01 and *** p < 0.001. (C) Distance moved during the visual motor response test. Data represent the mean distance moved during 10 min assay and is expressed as mean ± SD from five independent replicates (5 eleutheroembryo assayed for each treatment). Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple-comparison test. Different lowercase letters indicate significant differences between groups (p < 0.05).