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. 2021 Jan 8;11(1):18. doi: 10.3390/bios11010018

Figure 2.

Figure 2

Effect of the incubation of aptamer 40 nt with FB1 on the A650/520 ratio in (a) Tris-HCl buffer 31.1 mM, PBS 12.79 mM (NaCl equivalence) and a combination of both (Mix) to a final concentration of 31.1 (Tris-HCl) and 12.79 mM (PBS in NaCl equivalence), under the same binding conditions (n = 6), and (b) different buffers at the conditions outlined in Table 1 (the numbers correspond to each assay, n = 3). (c) Specificity of assay 4 incubated with other mycotoxins (13.8 µM, n = 4) and (d) the effect of the reduction in the aptamer: AuNP molar ratio (as shown in the legend subscripts) in the incubation with FB1 (F) and OTA (O)(n = 4).