Table 3.

Equations and LOD for aptamer-based quantification of FB1 with aptamer 96 nt through different signals ¹.

Signal		Equation	r2	Range of Tested Concentrations (µg/mL)	LOD (µg/mL)
A650/520		A650/520 = −0.074 ln [X] + 0.5153	0.9179	0.01–10	0.003
Absolute Area		AA = 1.34 ln [X] + 8.298	0.9243	0.01–10	0.002
Peak Area 520 nm	Peak 1	Area = 0.0002 ln [X] + 0.0036	0.9763	0.001–10	1.68
	Peak 2	Area = − 0.0005 ln [X] + 0.0065	0.8735	0.001–10	0.0001
Peak Area 600 nm	Peak 1	Area = 0.0017 ln [X] + 0.0334	0.872	0.001–10	7.83
	Peak 2	Area= − 0.006 ln [X] + 0.0946	0.858	0.001–10	0.000000056
Peak 2 Area /Peak 1 Area	520 nm	P1/P2 = − 0.268 ln [X] + 1.8427	0.7639	0.001–10	0.007
	600 nm	P1/P2 = − 0.364 ln [X] + 2.9186	0.9637	0.001–10	0.0006
Peak 2 Area–Peak 1 Area	600 nm	P–P2 = − 0.008 ln [X] + 0.059	0.9525	0.001–10	0.0000016
28o		Area = − 0.07 ln [X] + 0.4632	0.9128	0.001–10	0.00000016
Diameter		D = − 9.498 ln [X] + 124.61	0.9514	0.001–10	0.000959

¹ X = FB1concentration in µg/mL. Buffer: MgCl₂ 1mM; FB1 aptamer 96 nt incubation: 30 min (37 °C); incubation with AuNP: 60 min (RT); AuNP:Aptamer molar ratio 30:1.