Figure 2.

Validation of the direct repeat structures of Selaginella tamariscina via PCR and long-read sequencing. (a) Primers 1–6 were designed based on the junction regions between the single copies and repeat regions (the primer sequences are listed in Appendix A Table A1). The primers were used in various combinations to amplify hypothetical direct repeat structures and indirect repeat structures. (b) 1% agarose gel electrophoresis of the PCR products. (c) Confirmation of the direct repeat structure in assembled contigs spanning single-copy regions and the repeat region using Oxford Nanopore Sequencing technology.