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. 2021 Jan 12;10(1):135. doi: 10.3390/cells10010135

Figure 4.

Figure 4

SOX2 overexpression in radiosensitive colorectal cancer cells facilitated the induction of colorectal CSCs following irradiation. (A) Immunoblotting of SOX2 and CD44 in SOX2-overexpressing radiosensitive colorectal cancer cells (SW480 and LoVo) on day 2 after irradiation with 10 Gy. (B) Analysis of CD44+ cell population (left panel) and apoptotic cells (right panel) by flow cytometry in SOX2-overexpressing SW480 and LoVo cells. Data are shown as mean ± SD (n = 3). * p < 0.05 compared pcDNA + IR to WT-SOX2 + IR. (C) Colony formation assay was performed with SOX2-overexpressing SW480 and LoVo cells, and graph showing the quantification of relative colony numbers at different doses of radiation. Data are shown as mean ± SD (n = 3). (D) The images of migration and invasion on day 2 after radiation (10 Gy) of SOX2-overexpressing SW480 and LoVo cells were quantified. Bars indicate the measurements of migration and invasion. * p < 0.05 compared pcDNA + IR to WT-SOX2 + IR. (E) Tumoursphere-formation assay was performed to evaluate self-renewal of CSCs in SOX2-overexpressing SW480 and LoVo cells. Indicated cells were seeded in a non-adherent culture condition. After culturing for 7 days, the images and size of tumoursphere cells were analyzed. Data are shown as mean ± SD (n = 3). * p < 0.05 versus Cont.