Table 2.

Alignment of full tRNA reads dataset to manually curated tRNA references (Step 3).

Organism	tRNA Gene Number	% of Aligned Reads	Uniquely Aligned Reads 1	Multiply Aligned Reads 1
Escherichia_coli_str_K-12_substr_MG1655	39	95.87 ± 0.26	95.95 ± 0.23	4.05 ± 0.23
Bacillus_subtilis_subsp_subtilis_str_168	33	94.89 ± 0.91	93.93 ± 0.38	6.07 ± 0.38
Saccharomyces_cerevisiae_S288c	38	84.93 ± 6.61	98.52 ± 0.27	1.48 ± 0.27
Arabidopsis_thaliana_TAIR108feb2011	43	85.93 ± 2.34	94.08 ± 0.93	5.92 ± 0.93
Drosophila_melanogaster_BDGP6_dm6	34	83.67 ± 2.36	86.88 ± 2.36	13.12 ± 2.36
Homo_sapiens_GRCh37hg19	43	90.08 ± 0.70	89.15 ± 0.92	10.85 ± 0.92

¹ Normalized to the total number of aligned reads.