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. 2021 Jan 12;22(2):684. doi: 10.3390/ijms22020684

Figure 1.

Figure 1

A summary of commonly used methods to characterize exosomes: exosome uptake assay [15], exosome effect on irradiated-cell viability [15], SiRNA transfection [15], immunoblotting [15], cell surface expression analysis (flow cytometry) [15], MSC markers colocalization assay [15], exosome binding and colocalization assay [15,16,17], image acquisition details [18,19,20], apoptosis assay [19], quantitative reverse transcriptase polymerase chain reaction assay (RT-qPCR) [21], nanoparticle tracking analysis (NTA) [21,22,23], dynamic light scattering (DLS) analysis [16], Western blot analysis [24], relative quantitative mass spectrometry and nano-liquid chromatography mass spectrometry (nLC-MS) [21], and ELISA [25].