Figure 4.

In vitro assessment of silver nanocolloids loaded with betulinic acid effect on human hepatocellular carcinoma (HepG2) cells viability after 24- and 48-h treatment by applying the Alamar blue assay. The data are presented as cell viability percentage (%) normalized to control cells (a) and to DMSO, SilCo and PEG_SilCo, respectively (b) and are expressed as mean values ± SD of three independent experiments performed in triplicate. To identify the statistical differences between the control and the treated group, one-way ANOVA analysis was conducted, followed by Tukey’s multiple comparisons post-test (*** p < 0.001 and **** p < 0.0001).