Figure 5.

Improved LuTate uptake and growth suppression in BON-1 cells treated in sequence with chemotherapeutic drugs and LuTate. (A) LuTate uptake: BON-1 cells treated for 24 h with specified dose of three different drugs (or control) were allowed to recover in fresh medium for 4 days before exposure to LuTate for 4 h followed by processing for total cellular uptake of LuTate. The cells were seeded in triplicate and data derived from three independent experiments is presented as Mean ± SE. All three chemotherapy treatment groups had a statistically significant difference in LuTate uptake as compared to mock treated control. (B) Cell proliferation: An independent set of cells were treated with same regime but were allowed to recover for five days in fresh medium after removal of LuTate, and assessed for the fold increase in viable cell count to determine the rate of proliferation after initial seeding. The data derived from triplicates from two experiments are expressed as Mean ± SE. The * refers to a statistically significant difference (p < 0.05).