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. 2021 Jan 8;13(2):209. doi: 10.3390/cancers13020209

Figure 7.

Figure 7

Distinct expression of Piwi-interacting RNAs in EVs from LM patient CSF. Analysis of relative Piwi-interacting RNA (piRNA) expression profile in EVs extracted from LMs and HCs. Hierarchical clustering analysis of significantly expressed piRNA was visualized via (A) heatmap showing z score of extravesicular piRNA from HC (n = 2) and patients with LM (n = 8) with 54 piRNA satisfying FC2 and raw p-value. (B) Count of up- and downregulated piRNA was found by fold change and raw p-value. (C) Volcano plot shows differentially expressed piRNA in HC and LM patients with the x-axis showing log2 fold-change and y-axis showing -log10 of the raw p-value from LM versus HC piRNA expression counts. (D) Table displays the fold change of two piRNA in LM compared to HC ranked in the order of abundance. (E) Level of the two piRNAs was confirmed in HC (n = 3) and LM EVs (n = 8) using ddPCR (piRNA-36340: Mann-Whitney U test, * p < 0.05; piRNA-33415: Unpaired t-test with Welch’s correction, ** p < 0.01).