Figure 3.
LSM and TEM images of antibody labeling at 2, 4, and 24 h. Continuing deposition of the polysaccharides at the 2 h (A–G), 4 h (H–K), and 24 h (L–R) time points. Cellulose (A,H,L), MLG (B,E,I,M,P), and AX (C,F,J,N,Q) all continued to be deposited throughout the time course studied and visibly accumulated at the walls (arrows in TEM images). Clusters of MLG labeling (M; white arrows) are observed at 24 h, but these are punctate points at a higher magnification. Similar aggregations of filaments are present for AX (N; white arrows). Interestingly, callose deposition (D,G,K,O,R) appeared not to increase, but stayed fairly constant throughout the time course. Scale bars = 5 µm (A–D,H–O) and 200 nm (E–G,P–R).