Table 1.

The stain, carbohydrate binding module, and antibodies used to detect the various polysaccharides in the Lolium SCC cell wall.

Cell Wall Component	Name of Stain/Antibody	Specific Epitope	Source	Ref	Working Dilution
General cell wall stain	Calcofluor White	(1,3)-, (1,3;1,4)-, and (1,4)-β-glucans	Sigma	[41]	0.01%
Cellulose	CBM3a	crystalline cellulose	Plant Probes	[42]	1:50
Mixed-linkage glucan (MLG)	(1-3;1-4)-β-glucan-directed monoclonal antibody	linear (1,3;1,4)-β-oligo-saccharide segments in (1,3;1,4)-β-glucans	Biosupplies	[43]	1:500
Callose	(1-3)-β-glucan-directed monoclonal antibody	linear (1,3)-β-oligosaccharide segments in (1,3)-β-glucan	Biosupplies	[45]	1:300
Xylan	LM10	(1→4)-β-D-xylan	Plant Probes	[44]	1:20
Arabinoxylan	LM11	(1→4)-β-D-xylan/arabinoxylan	Plant Probes	[44]	1:20
Xyloglucan	LM15	XXXG motif of xyloglucans	Plant Probes	[50]	1:20
Mannan	(1-4)-β-mannan-directed monoclonal antibody	(1,4)-β-manno-oligosaccharides in (1,4)-β-mannans and galactomannans	Biosupplies	[51]	1:300
Pectin	JIM 7	homogalacturonan partially Me-HG, general pectin	Plant Probes	[52]	1:20
Arabinogalactan protein (AGP)	MAC207	AGP glycan	CCRC	[53]	1:10
Ferulic acid	NH4OH bathychromatic shift	Ferulic acid	Sigma	[54]	0.1 M