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. 2021 Jan 12;13(2):263. doi: 10.3390/cancers13020263

Figure 6.

Figure 6

DC-STAMP Expression is Reduced in Pre-Osteoclasts Exposed to EO CM. RAW 264.7 pre-osteoclasts were exposed to naïve OB CM (control), EO-231 CM, or EO-MCF-7 CM in the presence of 50 ng/mL exogenous RANKL for (A) three days, or (B) six days. RAW 264.7 pre-osteoclasts maintained in vehicle media (VM) in the presence of 50 ng/mL exogenous RANKL for (A) three days, or (B) six days served as additional controls. Uncropped Western Blots are available in Figures S6 and S7. Cell lysates were collected and subjected to western blot for the osteoclast fusion factor, dendrocyte expressed seven transmembrane protein (DC-STAMP; black arrow). The red arrow indicates 75 kDa, 50 kDa, and 37 kDa, respectively. β-actin (black arrow) served as a loading control. Values shown are band densitometry measurements for DC-STAMP protein expression, which was normalized to β-actin protein expression. For day 3, DC-STAMP protein expression densitometry values, as normalized to β-actin protein expression, were 1.468 (+VM; control), 1.295 (+Naïve OB CM; control), 1.328 (+EO-231 CM), and 1.442 (+EO-MCF-7 CM). For day 6, DC-STAMP protein expression densitometry values, as normalized to β-actin protein expression, were 1.174 (+VM; control), 1.342 (+Naïve OB CM; control), 1.282 (+EO-231 CM), and 1.086 (+EO-MCF-7 CM).