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. 2021 Jan 12;22(2):696. doi: 10.3390/ijms22020696

Figure 7.

Figure 7

AOH modulates the adhesion of 184A1 cells. (A) The results of the cell culture inserts are expressed as mean ± SE. (B) Representative results of stained cells after invasion assay, magnitude 40×. (C) The results of cells adhesion to ECM proteins are expressed as mean ± SE. (D) RTqPCR was performed to analyze the expression of MMP-2 and MMP-9. (E) zymography assay was performed to analyze the expression of MMP-2 and MMP-9. The results are expressed as mean ± SE. One-way ANOVA was used to calculate the statistical significance, p < 0.05 was established as significant. * p < 0.05, ** p < 0.01, *** p < 0.001 as compared to control. AOH–alternariol, E2–10 nM estradiol, Cnt–control, ECM–extracellular matrix, MMP-2–metalloproteinase 2, MMP-9–metalloproteinase 9.