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. 2021 Jan 12;13(2):262. doi: 10.3390/cancers13020262

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Enrichment of SOX2 is most consistent across ovarian cancer cell lines and contributes to spheroid formation efficiency. (A–C) qRT-PCR of selected genes relative to GAPDH housekeeping gene in cells grown for 5 days in 3-D conditions compared to 2-D conditions for (A) SOX2 (B) OCT4 (C) NANOG, (D–F) spheroid formation with inhibition of SOX2. Spheroids were measured after growth in 3-D conditions for 4 days after siRNA knockdown of SOX2 in (D) OV90 cells, (E) CAOV4 cells and (F) OVCAR8 cells. Student’s T-test 3-D vs 2-D or siSOX2 vs siNeg. Data represent mean and SEM. * p < 0.05.