Figure 5.

SOX2 expression correlates with tumor-initiation capacity (A) Limiting dilution assay comparing early and late appearance of subcutaneous xenograft tumors created with OV90 or ACI23 cells. (B–C) Time to tumor-initiation is dependent on cell dilution, One-way ANOVA, Tukey Post-hoc test. Early (open circles) and late (closed circles) appearing tumors were significantly different, Two-way ANOVA, Bonferroni post-hoc test. (D) Representative images of palpable ACI23 tumors that were resected, fixed, and histologically stained for SOX2, OCT4 or NANOG. (E) SOX2, OCT4 and NANOG staining was quantified from three mice from each group using ImageJ and four technical replicates to calculate digital histology score (Hscore), Two-way ANOVA, Bonferroni post-hoc test. Data represent mean and SEM. * p < 0.05.