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S. mutans prepared by conventional VP-SEM procedure. (a) VP-SEM 2000×. An intricate micro-canalicular system develops among bacterial towers. Image was captured from the same sample observed in [50] Fig. (b) VP-SEM 3000×. Hydration preservation confers biofilm a spongy aspect, without shrinking or any sign of collapse. Image was captured from the same sample observed in [50] Figure 2d.
