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. 2021 Jan 12;10(1):143. doi: 10.3390/cells10010143

Figure 3.

Figure 3

Epigenetic changes in the brain at 12 weeks after injury. Representative immunofluorescent images of double staining of (a) 5mC (green) and NeuN (red) and (b) 5hmC (green) and NeuN (red) in the primary motor cortex of the control and exercise groups. (c) 5mC and (d) 5hmC intensity in neurons in the primary motor cortex of the control and exercise groups (n = 4 per group). (e) Representative dot blot results for the primary motor cortex with 100, 250, and 500 ng of 5mC and 5hmC in the control and exercise groups. Quantitative data from dot-blot analysis of 250 ng of (f) 5mC and (g) 5hmC compared with the amount in total genomic DNA in the control and exercise groups. * p < 0.05 between the control and exercise groups by the Mann–Whitney U test. White scale bars = 50 µm.