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Analysis of complex formation between PsLYK9-ECD tagged with FLAG and PsLYR3-ECD, PsLYR4-ECD, or PsSYM10-ECD tagged with HIS determined by a co-immunoprecipitation assay. Extracellular domains of PsLYK9, PsLYR3, PsLYR4, or PsSYM10 were synthesized in E. coli. After extraction and incubation of the proteins on ice for 1 h, proteins were subjected to immunoprecipitation with anti-HIS resin and analyzed using immunoblotting with anti-FLAG and anti-HIS.
