Table A2.
HPLC-MS/MS validation data: Precision and recovery.
| Compound | Intraday and Interday Precision 1 (RSD) | Recovery 2 [%] | ||||
|---|---|---|---|---|---|---|
| Low Level [%] | High Level [%] | |||||
| Intraday | Interday | Intraday | Interday | Mean | SD | |
| α-KBA | 5.1 | 1.6 | 5.1 | 6.3 | 97.2 | 4.1 |
| β-KBA | 6.0 | 2.7 | 5.4 | 3.5 | 98.9 | 4.1 |
| α-AKBA | 5.2 | 7.5 | 2.6 | 7.6 | 90.9 | 4.6 |
| β-AKBA | 4.3 | 4.8 | 3.1 | 8.7 | 98.4 | 3.2 |
1 RSD: relative standard deviation [%]; low level: 50 ng/mL; high level: 500 ng/mL; intraday repetitions: n = 5; interday repetitions: n = 4. 2 Method of standard addition; sample # 25 (500 µg/mL) spiked on three levels (125, 250, and 500 ng/mL).