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. 2021 Jan 12;22(2):713. doi: 10.3390/ijms22020713

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of suramin on neural progenitor cell proliferation. Neural progenitor cells of the postnatal rat subventricular zone were cultured for 72 h in the absence (control) or presence of the purinergic receptor blocker suramin, and the percentage of bromodeoxyuridine (BrdU) incorporation during the last 12 h of culture was evaluated. Epifluorescence images showing BrdU immunohistochemistry (in green) in neurosphere-derived cells obtained from control cultures and cultures treated with 200 μM suramin. The total number of cells in each field was identified by 4′-6′-diamidino-2-phenylindole (DAPI) staining (in blue). Bar: 50 μm. The graph shows the percentage of BrdU-positive cells in each experimental condition. Data are the mean ± SEM (n = 6, p > 0.05, Mann–Whitney Rank Sum Test).