Figure 4.

Effect of suramin treatment on neural progenitor cell apoptosis. Neural progenitor cells from the postnatal rat subventricular zone were cultured in the absence (control) or presence of 200 μM suramin, and the percentage of apoptotic cells was evaluated by the TUNEL assay after 72 h of culture. Epifluorescence images showing apoptotic cells (in red) in control cultures and in cultures treated with suramin. The total number of cells in each field was identified by DAPI staining (in blue). Bar: 50 μm. The graph shows the percentage of apoptotic cells in each experimental condition. Data are mean ± SEM (n = 8, * p < 0.05, Student’s t-test).