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Binding efficiency on spheroids containing M21 human melanoma cells plus endothelial cells. Both M21 melanoma and HUVEC endothelial cells express the avß3 and NRP1 receptors. The binding efficiency of each type of SiNPs on the mixed spheroids was evaluated by fluorescence microscopy after excitation at 633 nm and with an emission filter from 640 to 740 nm, using a 10x objective.
